Citrulline

Citrulline is a non-essential amino acid recognized for its participation in the urea cycle, helping to manage nitrogen waste. Studies look at how it may enhance exercise capacity, support optimal blood flow, and contribute to the body’s natural detoxification pathways.

Chemical Name: L-Citrulline
CAS Number: 372-75-8
Molecular Formula: C6H13N3O3
Molecular Weight: 175.19 g/mol

With a purity exceeding 90%, Citrulline is suitable for use as a reference material or standard. It is available in convenient packaging sizes to support various laboratory needs.

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Synonym:

No synonym available.

Formula:

No formula available.

CAS Number:

372-75-8

Molecular Weight:

No molecular weight available.

Purity:

≥90%

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Using HPLC Standards with an Autosampler

Preparation:

Dilute your HPLC standards to the desired concentrations using a compatible solvent (generally bacteriostatic water for all water soluble compounds)

Loading the Autosampler:

Transfer the prepared standards into HPLC vials with tight-sealing caps to prevent contamination or evaporation. Label the vials clearly for easy identification. Place the vials in the autosampler tray according to the sample sequence you have programmed.

Programming the Autosampler:

Input the details of the standards, such as vial position, volume to inject, and run order, into the HPLC software. Ensure the autosampler is programmed to perform any necessary rinsing steps to avoid cross-contamination.

Running the Standards:

Begin the calibration run, allowing the autosampler to inject the standards into the HPLC system automatically. Monitor the chromatogram to confirm the peaks align with the known retention times and concentrations of the standards.

Data Analysis:

Use the chromatograms generated from the standards to create a calibration curve. Compare the peak areas or heights to determine the concentration of analytes in your unknown samples.