Isoleucine

Isoleucine is a branched-chain amino acid known for its roles in muscle metabolism and immune function. Researchers consider how it might contribute to hemoglobin production and energy regulation, focusing on its impact on maintaining balanced physiological operations and promoting healthy growth.

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Synonym(s):

2-Amino-3-methylpentanoic acid

Formula:

C6H13NO2

CAS Number:

73-32-5

Molecular Weight:

131.17 g/mol

Purity:

Quantity:

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Using HPLC Standards with an Autosampler

Preparation:
Dilute your HPLC standards to the desired concentrations using a compatible solvent (generally bacteriostatic water for all water soluble compounds)

Loading the Autosampler:
Transfer the prepared standards into HPLC vials with tight-sealing caps to prevent contamination or evaporation. Label the vials clearly for easy identification. Place the vials in the autosampler tray according to the sample sequence you have programmed.

Programming the Autosampler:
Input the details of the standards, such as vial position, volume to inject, and run order, into the HPLC software. Ensure the autosampler is programmed to perform any necessary rinsing steps to avoid cross-contamination.

Running the Standards:
Begin the calibration run, allowing the autosampler to inject the standards into the HPLC system automatically. Monitor the chromatogram to confirm the peaks align with the known retention times and concentrations of the standards.

Data Analysis:
Use the chromatograms generated from the standards to create a calibration curve. Compare the peak areas or heights to determine the concentration of analytes in your unknown samples.

Description

Chemical Name: L-Isoleucine
CAS Number: 73-32-5
Molecular Formula: C6H13NO2
Molecular Weight: 131.17 g/mol

With a purity exceeding 90%, Isoleucine is suitable for use as a reference material or standard. It is available in convenient packaging sizes to support various laboratory needs.

Additional Information

Synonym(s):

2-Amino-3-methylpentanoic acid

Formula:

C6H13NO2

CAS Number:

73-32-5

Molecular Weight:

131.17 g/mol

Purity:

Laboratory Tests

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